Visna-Maedi is an infectious dissease, diagnosed fort he first time in Iceland, due to a virus included in the family of Retroviridae. The disease is spreads prevalently among sheep that die after respiratory or nervuos sintomatology. Nowaday there is no effective prophylactics defences against the disease fort he extremly genetic bariability of virus. To achieve a thorough knowledge of epidemiologic situation in the Central Italy, it was carried out a sierologics survey on 131 flocks, 61 from Marche Region and 70 in Umb a, for a total of 1703 sera, It was utilized a immunoenzimathic test (ELISA) using as antigen a ricombinant protein gp25-gp44, obtained through biomolecolar procedures. Among 1703 sera, 970 were serologically positive for MVV. About the flocks 120 showed antibodies against MVV with a positivity percentage of 92. The positive flocks were respectively 64, with 92% in the district of Perugia and 56, with the 91 per cent in the area of Macerata.
Visna-Maedi: use of p25-gp44 in a immunoenzymatic test for serological diagnosis.
PREZIUSO, Silvia;CUTERI, Vincenzo;
2004-01-01
Abstract
Visna-Maedi is an infectious dissease, diagnosed fort he first time in Iceland, due to a virus included in the family of Retroviridae. The disease is spreads prevalently among sheep that die after respiratory or nervuos sintomatology. Nowaday there is no effective prophylactics defences against the disease fort he extremly genetic bariability of virus. To achieve a thorough knowledge of epidemiologic situation in the Central Italy, it was carried out a sierologics survey on 131 flocks, 61 from Marche Region and 70 in Umb a, for a total of 1703 sera, It was utilized a immunoenzimathic test (ELISA) using as antigen a ricombinant protein gp25-gp44, obtained through biomolecolar procedures. Among 1703 sera, 970 were serologically positive for MVV. About the flocks 120 showed antibodies against MVV with a positivity percentage of 92. The positive flocks were respectively 64, with 92% in the district of Perugia and 56, with the 91 per cent in the area of Macerata.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.