Can the fatty acids profile of Tuber aestivum - T. uncinatum species complex have chemotaxonomic value?

Fatty acid (FA) profiles are considered as chemotaxonomic markers to define groups of various taxonomic ranks in bacteria, yeasts, and fungi. Tuber aestivum is one of the most common European truffles that has environmental and economic values. While genetic studies suggest that T. aestivum Vittad. and T. uncinatum Chatin are synonymous, there is still debate over whether this is a species complex. In this work, we evaluate the differences in the total and individual FAs content of T. aestivum and T. uncinatum morphotypes, and T. melanosporum n-hexane extracts. The higher amounts of total linoleic acid and total oleic acid are found in T. uncinatum and characterize it from the T. aestivum. The composition of FA profiles of T. uncinatum-T. aestivum were distinguished by the presence of free palmitoleic acid (a chemotaxonomic marker) in T. uncinatum and absent in T. aestivum. Cluster analysis indicated that there were two groups for the FA profiles of the Tuber spp. hexanic extract: T. aestivum extract and T. uncinatum and T. melanosporum extracts. Our results indicate that T. aestivum and T. uncinatum have to be considered as two different taxa within the T. aestivum species complex.