Fast in situ forming thermosensitive hydrogels consisted of vinyl sulfone bearing p(HPMAm-lac1-2)-PEG-p(HPMAm-lac1-2) triblock copolymers and thiol modified hyaluronic acid were prepared via a dual cross-linking strategy based on thermal gelation at 37 °C and simultaneous Michael addition cross-linking between vinyl sulfone and thiol moieties. The formation of a chemical network was varied within a time period of 9-60 min by controlling the degree of vinyl sulfone derivatization, the triblock copolymer concentration and the degree of thiolation. The extent of thiol substitution on the polysaccharidic hyaluronan chain markedly affected the physical and mechanical properties, as well as the swelling and degradation behavior of the resulting networks, as confirmed by rheology, water uptake experiments and degradation tests. In addition, the developed hydrogels showed a good cytocompatibility in vitro during a timeframe of 21 days both for mouse bone marrow stromal cell and for NIH 3T3 mouse fibroblasts. The developed hydrogels showed potential as promising injectable biomaterials with tunable gelation kinetics, adjustable mechanical properties, swelling and degradation times. These biomaterials could find application both as a regenerative cell matrix and as controlled drug delivery system.

Injectable hyaluronic acid/PEG-p(HPMAm-lac)-based hydrogels dually cross-linked by thermal gelling and Michael addition

DUBBINI, ALESSANDRA;CENSI, Roberta;Butini, Maria Eugenia;SABBIETI, Maria Giovanna;AGAS, DIMITRIOS;DI MARTINO, Piera
2015-01-01

Abstract

Fast in situ forming thermosensitive hydrogels consisted of vinyl sulfone bearing p(HPMAm-lac1-2)-PEG-p(HPMAm-lac1-2) triblock copolymers and thiol modified hyaluronic acid were prepared via a dual cross-linking strategy based on thermal gelation at 37 °C and simultaneous Michael addition cross-linking between vinyl sulfone and thiol moieties. The formation of a chemical network was varied within a time period of 9-60 min by controlling the degree of vinyl sulfone derivatization, the triblock copolymer concentration and the degree of thiolation. The extent of thiol substitution on the polysaccharidic hyaluronan chain markedly affected the physical and mechanical properties, as well as the swelling and degradation behavior of the resulting networks, as confirmed by rheology, water uptake experiments and degradation tests. In addition, the developed hydrogels showed a good cytocompatibility in vitro during a timeframe of 21 days both for mouse bone marrow stromal cell and for NIH 3T3 mouse fibroblasts. The developed hydrogels showed potential as promising injectable biomaterials with tunable gelation kinetics, adjustable mechanical properties, swelling and degradation times. These biomaterials could find application both as a regenerative cell matrix and as controlled drug delivery system.
2015
File in questo prodotto:
File Dimensione Formato  
Eur Pol J 2015.pdf

solo gestori di archivio

Tipologia: Versione Editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 2.26 MB
Formato Adobe PDF
2.26 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11581/389206
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 29
  • ???jsp.display-item.citation.isi??? 28
social impact