The antioxidant capacity of capsules containing blueberry based products which are included among the group of integrators owing to their antioxidant capacity and produced by various films was investigated. The results of the investigation are compared to rank these products in order to their antioxidant capacity. In order to measure antioxidant capacity, our laboratory has recently developed a special electrochemical method based on a superoxide dismutase (SOD) biosensor to determine the superoxide radical. The results obtained by applying the SOD biosensor method to various blueberry based integrators were compared with the results obtained with the spectrophotometric (FRAP) method based on N,N-dimethyl-p-phenylendiamine (DMPD-FeCl3) and with those obtained also using the ORAC fluorimetric (TRAP) method. One of the more interesting aspect of the article is the good agreement it evidences of the results of the three methods for measuring antioxidant capacity. The three methods differ among themselves: an Electron Transfer (ET) method, a Hydrogen Atom Trasnfer Metod (HAT) and an electrochemical based biosensor method of the Morning Superoxide Radical (MSR) type. It is also shown how the antioxidant capacity of the fresh vegetable is in any case always greater then of any food supplement obtained from the same type of vegetable.
A superoxide dismutase biosensor for measuring the antioxidant capacity of blueberry based integrators
GABBIANELLI, Rosita;
2013-01-01
Abstract
The antioxidant capacity of capsules containing blueberry based products which are included among the group of integrators owing to their antioxidant capacity and produced by various films was investigated. The results of the investigation are compared to rank these products in order to their antioxidant capacity. In order to measure antioxidant capacity, our laboratory has recently developed a special electrochemical method based on a superoxide dismutase (SOD) biosensor to determine the superoxide radical. The results obtained by applying the SOD biosensor method to various blueberry based integrators were compared with the results obtained with the spectrophotometric (FRAP) method based on N,N-dimethyl-p-phenylendiamine (DMPD-FeCl3) and with those obtained also using the ORAC fluorimetric (TRAP) method. One of the more interesting aspect of the article is the good agreement it evidences of the results of the three methods for measuring antioxidant capacity. The three methods differ among themselves: an Electron Transfer (ET) method, a Hydrogen Atom Trasnfer Metod (HAT) and an electrochemical based biosensor method of the Morning Superoxide Radical (MSR) type. It is also shown how the antioxidant capacity of the fresh vegetable is in any case always greater then of any food supplement obtained from the same type of vegetable.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
