The base pair fluctuations and helix untwisting are examined for a circular molecule. A realistic mesoscopic model including twisting degrees of freedom and bending of the molecular axis is proposed. The computational method, based on path integral techniques, simulates a distribution of topoisomers with various twist numbers and finds the energetically most favorable molecularconformation as a function of temperature. The method can predict helical repeat, openings loci and bubble sizes for specic sequences in a broad temperature range. Some results are presented for a short DNA circle recently identied in mammalian cells.
Helix untwisting and bubble formation in circular DNA
ZOLI, Marco
2013-01-01
Abstract
The base pair fluctuations and helix untwisting are examined for a circular molecule. A realistic mesoscopic model including twisting degrees of freedom and bending of the molecular axis is proposed. The computational method, based on path integral techniques, simulates a distribution of topoisomers with various twist numbers and finds the energetically most favorable molecularconformation as a function of temperature. The method can predict helical repeat, openings loci and bubble sizes for specic sequences in a broad temperature range. Some results are presented for a short DNA circle recently identied in mammalian cells.File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
