This research examined the effects of dietary α-tocopheryl acetate (50 or 200 mg/kg diet) and selenium (Se, 0 or 0.5 ppm) supplementation on motion characteristics, oxidative stability and fertilizing ability of rabbit spermatozoa, fresh and stored for 24 h at 5°C. The higher amount of dietary α-tocopheryl acetate increased the level of Vitamin E in the fresh semen (1.75 mmol/l versus 0.95 mmol/l) and its oxidative stability (thiobarbituric acid reactive substances - TBARS 12.44 nmol malondialdehyde/108 sperm versus 21.4 nmol malondialdehyde/108 sperm). Dietary Se increased gluthatione peroxidase activity (GPx) in erythrocytes (285 U/g Hb versus 207 U/g Hb), seminal plasma (270 U/l versus 190 U/l) and spermatozoa (1338 mU/109 sperm versus 1103 mU/109 sperm), whereas it did not show any effect on α-tocopherol level and TBARS. No synergy between Vitamin E and Se was shown. Storage for 24 h at 5°C increased the TBARS level in all the experimental groups. Neither live and acrosome reacted spermatozoa, nor kinetic parameters, nor fertility rate were modified by dietary supplementation.
Effect of supranutritional level of dietary alpha-tocopheryl acetate and selenium on rabbit semen
BEGHELLI, Daniela
2002-01-01
Abstract
This research examined the effects of dietary α-tocopheryl acetate (50 or 200 mg/kg diet) and selenium (Se, 0 or 0.5 ppm) supplementation on motion characteristics, oxidative stability and fertilizing ability of rabbit spermatozoa, fresh and stored for 24 h at 5°C. The higher amount of dietary α-tocopheryl acetate increased the level of Vitamin E in the fresh semen (1.75 mmol/l versus 0.95 mmol/l) and its oxidative stability (thiobarbituric acid reactive substances - TBARS 12.44 nmol malondialdehyde/108 sperm versus 21.4 nmol malondialdehyde/108 sperm). Dietary Se increased gluthatione peroxidase activity (GPx) in erythrocytes (285 U/g Hb versus 207 U/g Hb), seminal plasma (270 U/l versus 190 U/l) and spermatozoa (1338 mU/109 sperm versus 1103 mU/109 sperm), whereas it did not show any effect on α-tocopherol level and TBARS. No synergy between Vitamin E and Se was shown. Storage for 24 h at 5°C increased the TBARS level in all the experimental groups. Neither live and acrosome reacted spermatozoa, nor kinetic parameters, nor fertility rate were modified by dietary supplementation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
